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### Author Topic: Calibration Question  (Read 955 times)

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#### DivideByZer0

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• Posts: 1
##### Calibration Question
« on: April 27, 2012, 05:28:56 PM »
Hi everyone,

First post and it seemed like there are some knowledgeable people here, I hope this is the right place to ask:

I'm working on a research project involving a microscope (connected to a camera; the video feed is streamed to an application we're developing) and a manipulator arm.  The microscope and manipulator arm are both controlled by a Luigs & Neumann control box (very obsolete - the computer interfaces with it with a serial cable and its response time is slowwww.)  The microscope can move in 3 dimensions; X, Y, and Z which are at right angles to one another.  The box can be queried, and will return decimal values for the position of each axis of each device.  Each device can be sent a command to move to a specific position, with sub-micrometer precision.

The manipulator arm, however, is adjustable in all 3 dimensions, and thus there is no guarantee that any of its axes are at right angles.  We need to be able to look at the video stream from the camera, and then click on a point on the screen where we want the tip of the manipulator arm to move to.  Thus, the two coordinate systems have to be calibrated.

Right now, we have achieved calibration by moving the microscope/camera's position to the tip of the manipulator arm, setting that as the synchronization point between the two coordinate systems, and moving the manipulator arm +250um in the X direction, moving the microscope to the tip of the manipulator arm at this new position, and then using the differences between these values to define a 3d vector that corresponds to the distance and direction moved by the manipulator, per unit in the microscope coordinate system.  This is repeated for each axis of the manipulator arm.

Once this data is obtained, in order to move the manipulator arm to a specific location in the microscope coordinate system, a system of equations can be solved by the program which determines how much it needs to move the manipulator in each axis to move it to the center point of the screen.  This works pretty reliably so far.

The issue we're running into here is that due to the slow response time of the equipment, it can take 5-10 minutes to complete the calibration process, which is complicated by the fact that the tip of the manipulator arm must be changed occasionally during an experiment, requiring the calibration process to be repeated.  Our research is rather time sensitive and this creates a major bottleneck in the process.

My linear algebra is a little patchy, but it seems like if we measure the units distance traveled by the tip of the manipulator arm per unit in the microscope coordinate system and have this just hard coded into the program (for now), it might be possible to move all 3 axes of the manipulator a specific amount at the same time, and then to derive the vectors for each axis from this information.  I'm not really sure how to go about doing this (or if it's even possible to do this), and any information or advice would be greatly appreciated.
« Last Edit: April 27, 2012, 05:50:09 PM by DivideByZer0 »