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BTW, i'm using vista x64 with the AVRISP mkII and AVRStudio 4.15 without any issues.
I don't think you can use avrdude and winavr mkII drivers at the same time, one shows up as Jungo, and the other just shows up as USB in your device manager
Now verify that the device selected is indeed your device. Click the "Settings" button right above ISP Frequency, and set your ISP frequency to 125 kHz.
It should be set to less than 1/4 of the Microcontroller's frequency , but I've found that around 1/8 of the microcontroller's frequency works best.
airman00, i'm surprised you are using 125 kHz. It really should be based on speed of your clock right? If your ATmega168 is running at 16 MHz, you can put the ISP to 2.0 MHz which is less than 1/4 of the frequency. I find the 125 kHz is too slow in programming the chip.